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Protein kinase A accelerates the rate of early stage differentiation of pluripotent stem cells.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Mar 26; Vol. 524 (1), pp. 57-63. Date of Electronic Publication: 2020 Jan 22. - Publication Year :
- 2020
-
Abstract
- In normal development, the rate of cell differentiation is tightly controlled and critical for normal development and stem cell differentiation. However, the underlying mechanisms regulating the rate of the differentiation are unknown, and manipulation of the rate of the stem cell differentiation is currently difficult. Here we show that activation of protein kinase A (PKA) accelerates the rate of mouse embryonic stem cell (ESC) differentiation through an early loss of ESC pluripotency markers and early appearance of mesodermal and other germ layer cells. The activation of PKA hastened differentiation by increasing the expression of a histone H3 lysine 9 (H3K9) dimethyltransferase, G9a protein, and the level of a negative epigenetic histone mark, H3K9 dimethylation (H3K9me2), in the promoter regions of the pluripotency markers Nanog and Oct4. These results elucidate a novel role of PKA on ESC differentiation and offer an experimental model for controlling the rate of ESC differentiation.<br />Competing Interests: Declaration of competing interest To the best of our knowledge, the authors have no conflict of interest, financial or otherwise.<br /> (Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Biomarkers metabolism
Cell Differentiation
Gene Expression Regulation, Developmental
Germ Layers metabolism
Histone-Lysine N-Methyltransferase metabolism
Methylation
Mice
Nanog Homeobox Protein metabolism
Octamer Transcription Factor-3 metabolism
Signal Transduction
Cyclic AMP-Dependent Protein Kinases metabolism
Mouse Embryonic Stem Cells metabolism
Pluripotent Stem Cells metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 524
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 31980180
- Full Text :
- https://doi.org/10.1016/j.bbrc.2019.12.098