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Progesterone induces sperm release from oviductal epithelial cells by modifying sperm proteomics, lipidomics and membrane fluidity.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2020 Mar 15; Vol. 504, pp. 110723. Date of Electronic Publication: 2020 Jan 20. - Publication Year :
- 2020
-
Abstract
- The sperm reservoir is formed after insemination in mammals, allowing sperm storage in the oviduct until their release. We previously showed that physiological concentrations of progesterone (P4) trigger in vitro the sperm release from bovine oviductal epithelial cells (BOECs), selecting a subpopulation of spermatozoa with a higher fertilizing competence. Here, by using Western-Blot, confocal microscopy and Intact Cell MALDI-TOF-Mass Spectrometry strategies, we elucidated the changes derived by the P4-induced release on sperm cells (BOEC-P4 spz). Our findings show that, compared to controls, BOEC-P4 spz presented a decrease in the abundance of Binder of Sperm Proteins (BSP) -3 and -5, suggesting one mechanism by which spermatozoa may detach from BOECs, and thus triggering the membrane remodeling with an increase of the sperm membrane fluidity. Furthermore, an interesting number of membrane lipids and proteins were differentially abundant in BOEC-P4 spz compared with controls.<br />Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest regarding the publication of this article.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cattle
Cells, Cultured
Epithelial Cells drug effects
Epithelial Cells metabolism
Fallopian Tubes metabolism
Female
Lipidomics
Male
Proteome metabolism
Proteomics
Spermatozoa metabolism
Fallopian Tubes drug effects
Membrane Fluidity drug effects
Progesterone pharmacology
Proteome drug effects
Sperm-Ovum Interactions drug effects
Spermatozoa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8057
- Volume :
- 504
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 31972329
- Full Text :
- https://doi.org/10.1016/j.mce.2020.110723