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Increased activity of the sterol branch of the mevalonate pathway elevates glycosylation of secretory proteins and improves antifungal properties of Trichoderma atroviride.
- Source :
-
Fungal genetics and biology : FG & B [Fungal Genet Biol] 2020 Apr; Vol. 137, pp. 103334. Date of Electronic Publication: 2020 Jan 17. - Publication Year :
- 2020
-
Abstract
- Some Trichoderma spp. have an ability to inhibit proliferation of fungal plant pathogens in the soil. Numerous compounds with a proven antifungal activity are synthesized via the terpene pathway. Here, we stimulated the activity of the mevalonate pathway in T. atroviride P1 by expressing the Saccharomyces cerevisiae ERG20 gene coding for farnesyl pyrophosphate (FPP) synthase, a key enzyme of this pathway. ERG20-expressing Trichoderma strains showed higher activities of FPP synthase and squalene synthase, the principal recipient of FPP in the mevalonate pathway. We also observed activation of dolichyl phosphate mannose (DPM) synthase, an enzyme in protein glycosylation, and significantly increased O- and N-glycosylation of secreted proteins. The hyper-glycosylation of secretory hydrolases could explain their increased activity observed in the ERG20 transformants. Analysis of the antifungal properties of the new strains revealed that the hydrolases secreted by the transformants inhibited growth of a plant pathogen, Pythium ultimum more efficiently compared to the control strain. Consequently, the biocontrol activity of the transgenic strains, determined as their ability to protect bean seeds and seedlings against harmful action of P. ultimum, was also improved substantially.<br /> (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Antifungal Agents metabolism
Fabaceae microbiology
Gene Expression Regulation, Fungal genetics
Geranyltranstransferase genetics
Geranyltranstransferase metabolism
Glycosylation
Hypocreales genetics
Mannosyltransferases genetics
Pythium growth & development
Sterols metabolism
Trichoderma genetics
Hypocreales metabolism
Mevalonic Acid metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0937
- Volume :
- 137
- Database :
- MEDLINE
- Journal :
- Fungal genetics and biology : FG & B
- Publication Type :
- Academic Journal
- Accession number :
- 31958566
- Full Text :
- https://doi.org/10.1016/j.fgb.2020.103334