Back to Search
Start Over
Scopoletin Activates Adenosine Monophosphate-Activated Protein Kinase/Mammalian Target of Rapamycin Signaling Pathway and Improves Functional Recovery after Spinal Cord Injury in Rats.
- Source :
-
Pharmacology [Pharmacology] 2020; Vol. 105 (5-6), pp. 349-359. Date of Electronic Publication: 2020 Jan 17. - Publication Year :
- 2020
-
Abstract
- Background: Scopoletin (SPT) is known to exert neuroprotective autophagy effect. However, the efficacy of SPT in the treatment of spinal cord injury (SCI) has yet not been explored. The investigation was intended to elucidate whether SPT can exert neuroprotective effect by triggering neuronal autophagy after SCI. The study was also directed to investigate the role of adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) signaling pathway in the autophagy facilitated by SPT.<br />Materials and Methods: The SCI was developed in female Sprague-Dawley rats by damaging the T10 spinal level using an impounder impact. Three animals groups were investigated - Sham group, SCI group, and SCI + SPT group. The SCI + SPT group was administered with SPT (100 mg/kg) intraperitoneally. Basso, Beattie, and Bresnahan scores and angle of incline test revealed that SPT administration improved the movement of hind limbs after SCI induction.<br />Results: Results indicated that SPT imparted neuronal protection, alleviated neuronal apoptosis, and improved neuronal autophagy. SPT-induced autophagy was identified by increased Beclin-1 expression and LC3B-positive neuronal cells. Further investigations revealed that SPT triggers the pathway involving AMPK/mTOR signaling, thereby stimulating autophagy in SCI-induced rat model.<br />Conclusion: The findings of the present investigation strongly advocate the beneficial effects of SPT in the treatment of the SCI. SPT ameliorates the AMPK/mTOR signaling-induced autophagy and thereby improves functional recovery in SCI-induced rats.<br /> (© 2020 S. Karger AG, Basel.)
- Subjects :
- Animals
Apoptosis drug effects
Autophagy drug effects
Female
Locomotion drug effects
Neurons drug effects
Neuroprotective Agents therapeutic use
Rats
Rats, Sprague-Dawley
Recovery of Function drug effects
Scopoletin therapeutic use
Signal Transduction drug effects
Spinal Cord drug effects
Spinal Cord Injuries metabolism
Spinal Cord Injuries pathology
Spinal Cord Injuries physiopathology
AMP-Activated Protein Kinases metabolism
Neuroprotective Agents pharmacology
Scopoletin pharmacology
Spinal Cord Injuries drug therapy
TOR Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0313
- Volume :
- 105
- Issue :
- 5-6
- Database :
- MEDLINE
- Journal :
- Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 31955175
- Full Text :
- https://doi.org/10.1159/000503866