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Single Neuron Imaging Reveals Metabotropic Glutamate Receptor-Mediated Bursting and Delay in Calcium Oscillation in Hippocampal Neurons.

Authors :
Singh R
Saxena A
Giri L
Source :
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference [Annu Int Conf IEEE Eng Med Biol Soc] 2019 Jul; Vol. 2019, pp. 5146-5149.
Publication Year :
2019

Abstract

Measurement of cytosolic calcium activity in single neuron and quantification of response to drug treatment over a longer period remain challenging. Especially, analysis of amplitude, frequency, and delay in calcium response in G-protein coupled receptors (GPCR) targeting drug-treated neurons is complicated due to inherent heterogeneity. To address this, we have utilized k- means clustering to identify various sub-populations. Here we focus on measuring drug dose response in the single neuron by means of high-resolution confocal imaging. Metabotropic glutamate receptor was activated using 3,5-dihydroxyphenylglycine (DHPG). The result reveals an unusual delay in calcium response for higher dose particularly in responding neurons. The proposed methodology can be used for selection of drug dose and optimization of the time window for neurodegenerative diseases.

Details

Language :
English
ISSN :
2694-0604
Volume :
2019
Database :
MEDLINE
Journal :
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Publication Type :
Academic Journal
Accession number :
31947017
Full Text :
https://doi.org/10.1109/EMBC.2019.8856638