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Detection of HER2 + Breast Cancer Cells using Bioinspired DNA-Based Signal Amplification.
- Source :
-
ChemMedChem [ChemMedChem] 2020 Apr 20; Vol. 15 (8), pp. 661-666. Date of Electronic Publication: 2020 Jan 16. - Publication Year :
- 2020
-
Abstract
- Circulating tumor cells (CTC) are promising biomarkers for metastatic cancer detection and monitoring progression. However, detection of CTCs remains challenging due to their low frequency and heterogeneity. Herein, we report a bioinspired approach to detect individual cancer cells, based on a signal amplification cascade using a programmable DNA hybridization chain reaction (HCR) circuit. We applied this approach to detect HER2 <superscript>+</superscript> cancer cells using the anti-HER2 antibody (trastuzumab) coupled to initiator DNA eliciting a HCR cascade that leads to a fluorescent signal at the cell surface. At 4 °C, this HCR detection scheme resulted in highly efficient, specific and sensitive signal amplification of the DNA hairpins specifically on the membrane of the HER2 <superscript>+</superscript> cells in a background of HER2 <superscript>-</superscript> cells and peripheral blood leukocytes, which remained almost non-fluorescent. The results indicate that this system offers a new strategy that may be further developed toward an in vitro diagnostic platform for the sensitive and efficient detection of CTC.<br /> (© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
Details
- Language :
- English
- ISSN :
- 1860-7187
- Volume :
- 15
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- ChemMedChem
- Publication Type :
- Academic Journal
- Accession number :
- 31943804
- Full Text :
- https://doi.org/10.1002/cmdc.201900697