Back to Search
Start Over
Interleukin-8-Induced Invasion Assay in Triple-Negative Breast Cancer Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2108, pp. 107-115. - Publication Year :
- 2020
-
Abstract
- The pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8) induces proliferation and invasion of solid tumor cells. In many types of solid cancer, including triple-negative breast cancer (TNBC), the IL-8 expression is induced by proteasome inhibition. In this chapter, we describe a protocol for the analysis of TNBC cell invasion induced by IL-8 in response to proteasome inhibition by bortezomib (BZ). Using this approach, we show that BZ increases the invasion ability of TNBC cells, and that the BZ-increased TNBC cell invasion is suppressed by IκB kinase (IKK) inhibition, which also decreases the IL-8 expression. The experimental protocol includes the cell invasion assay, microscopic evaluation of the invading cells, and quantitative analysis of the obtained images. This protocol should be applicable also for measurement of chemokine-induced tumor cell invasion in other types of cancer cells.
- Subjects :
- Biomarkers
Cell Line, Tumor
Cell Proliferation
Cytokines metabolism
Data Analysis
Female
Humans
I-kappa B Kinase metabolism
Interleukin-8 pharmacology
Molecular Imaging
Proteasome Inhibitors pharmacology
Software
Triple Negative Breast Neoplasms pathology
Cell Movement drug effects
Interleukin-8 metabolism
Triple Negative Breast Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2108
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31939174
- Full Text :
- https://doi.org/10.1007/978-1-0716-0247-8_9