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Role of Toll-like receptor 2 against Streptococcus uberis infection in primary mouse mammary epithelial cells.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2020 Feb; Vol. 79, pp. 106142. Date of Electronic Publication: 2020 Jan 10. - Publication Year :
- 2020
-
Abstract
- Mammary epithelial cells (MECs) play an important role against Streptococcus uberis infection which is one of the main causes of bovine mastitis and a potential threat to human health. Toll-like receptors (TLRs) and their mediated signaling pathways are critical in both innate and infection responses, yet their roles in anti-S. uberis infection in MECs remains poorly defined. In this work we investigated the regulatory mechanisms of TLR2 in inflammatory responses, where WT and TLR2 <superscript>-/-</superscript> mice were euthanized at 15-18 days gestation, and mammary gland tissues were collected aseptically. The mouse MECs (MMECs) were isolated by combined digestion with type I collagenase, hyaluronidase and trypsin. We challenged MMECs with S. uberis and quantified antioxidant capacity as well as reactive oxygen species (ROS), proinflammatory cytokines and cell damage at different times. The loss of TLR2 function in MMECs results in more serious cell damage, increased cell adhesion, and significantly decreased ROS and mitochondrial ROS (mROS) with bactericidal function in response to S. uberis infection. Moreover, it was observed that the antioxidant capacity declined, and the production of TLR2-mediated cytokines (except CXC ligand 15) also were reduced. We demonstrated that TLR2 can mediate cellular anti-infective processes in MMECs by regulating the production of ROS and mROS and the secretion of cytokines. The results suggest an unpredicted role of TLR2 in MMECs in response to S. uberis infection.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Apoptosis
Cells, Cultured
Epithelial Cells pathology
Humans
Mice
Mice, Inbred C57BL
Mice, Knockout
Oxidative Stress
Primary Cell Culture
Reactive Oxygen Species metabolism
Toll-Like Receptor 2 genetics
Epithelial Cells metabolism
Mammary Glands, Human pathology
Streptococcal Infections immunology
Streptococcus physiology
Toll-Like Receptor 2 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 79
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 31931293
- Full Text :
- https://doi.org/10.1016/j.intimp.2019.106142