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Monoclonal antibodies against LipL32 confer prophylactic protection against lethal leptospirosis challenge in animal model.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2020 Apr; Vol. 141, pp. 103975. Date of Electronic Publication: 2020 Jan 11. - Publication Year :
- 2020
-
Abstract
- Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira. The commercially available vaccines are bacterins that offer limited protection, short-term effect, and serovar-specific immunity. The development of novel immunization strategies is crucial to control the infection and decrease the chances of new outbreaks. In this study, purified monoclonal antibodies (mAbs) anti-LipL32 (1D9 and mAb3) were evaluated by their capacity to bind and neutralize the pathogen improving host survival. For that, an in vitro growth inhibition assay, and in vivo passive immunization were performed in animal model. Syrian hamsters were passively immunized by three different strategies. Hamsters immunized with mAb3 6 h prior to the lethal challenge showed a significantly higher survival rate of 61.1%, and a significant reduction in tissue damage in the lungs. Cumulatively, our results showed that anti-LipL32 mAbs inhibited the growth of L. interrogans in vitro, and that passive immunization offered significant protection in animal model when administered prior to infection.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Bacterial pharmacology
Antibodies, Monoclonal pharmacology
Cricetinae
Disease Models, Animal
Female
Immunization
Leptospirosis microbiology
Leptospirosis mortality
Leptospirosis pathology
Treatment Outcome
Antibodies, Bacterial immunology
Antibodies, Monoclonal immunology
Bacterial Outer Membrane Proteins immunology
Leptospira interrogans immunology
Leptospirosis prevention & control
Lipoproteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 141
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 31931114
- Full Text :
- https://doi.org/10.1016/j.micpath.2020.103975