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FilTar: using RNA-Seq data to improve microRNA target prediction accuracy in animals.

Authors :
Bradley T
Moxon S
Source :
Bioinformatics (Oxford, England) [Bioinformatics] 2020 Apr 15; Vol. 36 (8), pp. 2410-2416.
Publication Year :
2020

Abstract

Motivation: MicroRNA (miRNA) target prediction algorithms do not generally consider biological context and therefore generic target prediction based on seed binding can lead to a high level of false-positive predictions. Here, we present FilTar, a method that incorporates RNA-Seq data to make miRNA target prediction specific to a given cell type or tissue of interest.<br />Results: We demonstrate that FilTar can be used to: (i) provide sample specific 3'-UTR reannotation; extending or truncating default annotations based on RNA-Seq read evidence and (ii) filter putative miRNA target predictions by transcript expression level, thus removing putative interactions where the target transcript is not expressed in the tissue or cell line of interest. We test the method on a variety of miRNA transfection datasets and demonstrate increased accuracy versus generic miRNA target prediction methods.<br />Availability and Implementation: FilTar is freely available and can be downloaded from https://github.com/TBradley27/FilTar. The tool is implemented using the Python and R programming languages, and is supported on GNU/Linux operating systems.<br />Supplementary Information: Supplementary data are available at Bioinformatics online.<br /> (© The Author(s) 2020. Published by Oxford University Press.)

Details

Language :
English
ISSN :
1367-4811
Volume :
36
Issue :
8
Database :
MEDLINE
Journal :
Bioinformatics (Oxford, England)
Publication Type :
Academic Journal
Accession number :
31930382
Full Text :
https://doi.org/10.1093/bioinformatics/btaa007