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Origin and role of the cerebrospinal fluid bidirectional flow in the central canal.

Authors :
Thouvenin O
Keiser L
Cantaut-Belarif Y
Carbo-Tano M
Verweij F
Jurisch-Yaksi N
Bardet PL
van Niel G
Gallaire F
Wyart C
Source :
ELife [Elife] 2020 Jan 09; Vol. 9. Date of Electronic Publication: 2020 Jan 09.
Publication Year :
2020

Abstract

Circulation of the cerebrospinal fluid (CSF) contributes to body axis formation and brain development. Here, we investigated the unexplained origins of the CSF flow bidirectionality in the central canal of the spinal cord of 30 hpf zebrafish embryos and its impact on development. Experiments combined with modeling and simulations demonstrate that the CSF flow is generated locally by caudally-polarized motile cilia along the ventral wall of the central canal. The closed geometry of the canal imposes the average flow rate to be null, explaining the reported bidirectionality. We also demonstrate that at this early stage, motile cilia ensure the proper formation of the central canal. Furthermore, we demonstrate that the bidirectional flow accelerates the transport of particles in the CSF via a coupled convective-diffusive transport process. Our study demonstrates that cilia activity combined with muscle contractions sustain the long-range transport of extracellular lipidic particles, enabling embryonic growth.<br />Competing Interests: OT, LK, YC, MC, FV, NJ, PB, Gv, FG No competing interests declared, CW Reviewing editor, eLife<br /> (© 2020, Thouvenin et al.)

Details

Language :
English
ISSN :
2050-084X
Volume :
9
Database :
MEDLINE
Journal :
ELife
Publication Type :
Academic Journal
Accession number :
31916933
Full Text :
https://doi.org/10.7554/eLife.47699