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Impact of selective and non-selective media on prevalence and genetic makeup of ESBL/pAmpC-producing Escherichia coli in the broiler production pyramid.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2020 Jan; Vol. 240, pp. 108536. Date of Electronic Publication: 2019 Nov 30. - Publication Year :
- 2020
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Abstract
- Presence of extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (pAmpC)-producing Escherichia coli (ESBL/pAmpC-EC) in humans and animals is alarming due to the associated risks of antibiotic therapy failure. ESBL/pAmpC-EC transmission between the human and animal compartments remains controversial. Using cefotaxime-supplemented (selective) media, we recently showed high sample prevalence of ESBL/pAmpC-EC in an integrated broiler chain [i.e. Parent Stock (PS), offspring broilers and their carcasses]. Here, we used a different approach. In parallel with the selective isolation, samples were processed on non-selective media. E. coli isolates were tested for ESBL/pAmpC-production and those found positive were genotyped. For carcasses, total E. coli were enumerated. This approach enabled us to estimate prevalence at the isolate level, which mirrors ESBL/pAmpC-EC colonisation levels. We showed that although present in many animals, ESBL/pAmpC-EC were overall subdominant to intestinal E. coli, indicating that high sample prevalence is not associated with high levels of resistance in individual hosts. This is a relevant aspect for risk assessments, especially regarding the immediate exposure of farm personnel. An exception was a particularly dominant B2/bla <subscript>CMY-2</subscript> lineage in the gut of imported PS chicks. This predominance obscured presence of latent genotypes, however bias towards particular ESBL/pAmpC-EC genotypes from the selective method or underestimation by the non-selective approach did not occur. At the slaughterhouse, we showed a link between total E. coli and ESBL/pAmpC-EC on carcasses. Mitigation strategies for reducing consumers' exposure should aim at suppressing ESBL/pAmpC-EC in the broiler gut as well as controlling critical points in the processing line.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Abattoirs
Animals
Anti-Bacterial Agents pharmacology
Cefotaxime pharmacology
Chickens microbiology
Drug Resistance, Multiple, Bacterial
Escherichia coli drug effects
Escherichia coli enzymology
Farmers
Genotype
Humans
Intestines microbiology
Italy epidemiology
Poultry Diseases epidemiology
Prevalence
Bacterial Proteins genetics
Culture Media chemistry
Escherichia coli genetics
Escherichia coli growth & development
Escherichia coli Infections veterinary
Poultry Diseases microbiology
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 240
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31902501
- Full Text :
- https://doi.org/10.1016/j.vetmic.2019.108536