Back to Search
Start Over
Fabrication of a water-soluble near-infrared fluorescent probe for selective detection and imaging of dipeptidyl peptidase IV in biological systems.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2020 Jan 28; Vol. 8 (4), pp. 767-775. Date of Electronic Publication: 2020 Jan 03. - Publication Year :
- 2020
-
Abstract
- Dipeptidyl peptidase IV (DPP-IV) is a transmembrane glycoprotein known to regulate T cell activation, which is related to various pathological processes and has become a potential target to treat type 2 diabetes mellitus. Therefore, it is significant for the evaluation of endogenous DPP-IV activity in various biological systems. Herein, a water-soluble near-infrared (NIR) fluorescent probe HCA-D based on cyanine dyes as the fluorophore and glycyl-prolyl peptide as the specific recognition sequence was developed for the assay of dipeptidyl peptidase IV (DPP-IV) activity. Upon addition of DPP-IV, HCA-D can emit a significant turn-on NIR fluorescence signal under physiological conditions and exhibit high selectivity toward DPP-IV. This feature was available for quantifying DPP-IV in the range from 0.62 to 10 ng mL <superscript>-1</superscript> with a detection limit of 0.19 ng mL <superscript>-1</superscript> . Furthermore, the present probe was successfully employed for monitoring DPP-IV in serum samples from diabetic and healthy people, and imaging of DPP-IV in living cells and tumor mice models. These results demonstrate that the designed probe provides a promising tool to explore the relationship between DPP-IV and diabetes mellitus or other diseases. Perhaps, it may become a prospective image-guided tumor resection indicator based on the abnormal expression of DPP-IV activity in the future.
- Subjects :
- Animals
Cell Survival drug effects
Cells, Cultured
Dipeptidyl Peptidase 4 metabolism
Dipeptidyl-Peptidase IV Inhibitors pharmacology
Dose-Response Relationship, Drug
Fluorescent Dyes chemical synthesis
Fluorescent Dyes pharmacology
Humans
Infrared Rays
Liver Neoplasms, Experimental diagnostic imaging
Materials Testing
Mice
Mice, Inbred Strains
Molecular Structure
Optical Imaging
RAW 264.7 Cells
Sitagliptin Phosphate pharmacology
Solubility
Water chemistry
Dipeptidyl Peptidase 4 analysis
Fluorescent Dyes chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 31897456
- Full Text :
- https://doi.org/10.1039/c9tb02301a