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Determination of pathogenic bacteria-Bacillus anthrax spores in environmental samples by ratiometric fluorescence and test paper based on dual-emission fluorescent silicon nanoparticles.
- Source :
-
Journal of hazardous materials [J Hazard Mater] 2020 Mar 15; Vol. 386, pp. 121956. Date of Electronic Publication: 2019 Dec 23. - Publication Year :
- 2020
-
Abstract
- Many lanthanide ions-based probes have been widely used for detecting anthrax spores biomarker-dipicolinic acid (DPA). However, little work has realized detection of bacillus anthrax spores in real environmental samples. In this work, a novel ratiometric fluorescent nanoprobe based on europium (Eu)-doped silicon nanoparticles (Eu@SiNPs) was fabricated for the first time by one-pot method without post-modification for determination of the DPA in bacillus subtilis spores (simulant bacillus anthrax spores). Based on Eu(III) in the Eu@SiNPs could be sensitized by DPA to emit intrinsic fluorescence and the fluorescence intensity of SiNPs in the Eu@SiNPs almost remained stable, a new ratiometric fluorescent method for determination of micro DPA in bacillus subtilis spores and bacillus subtilis spores in real environmental samples, such as Yellow river water, tap water and soil was established. Under the optimum conditions, the limit of detection (LOD) of the method toward bacillus subtilis spores was as low as 2.38×10 <superscript>4</superscript> spore/mL. Simple, fast and visual DPA and bacillus subtilis spores determination was also achieved by the Eu@SiNPs-based test paper. Therefore, the newly established method was expected to be a powerful tool for efficiently determination of bacillus anthrax spores to avoid anthrax threats.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Bacillus anthracis pathogenicity
Limit of Detection
Microscopy, Fluorescence
Paper
Rivers microbiology
Bacillus anthracis physiology
Environmental Monitoring methods
Europium chemistry
Fluorescent Dyes chemistry
Nanoparticles chemistry
Propylamines chemistry
Silanes chemistry
Spores, Bacterial isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3336
- Volume :
- 386
- Database :
- MEDLINE
- Journal :
- Journal of hazardous materials
- Publication Type :
- Academic Journal
- Accession number :
- 31884372
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2019.121956