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Structural Insights Into PfARO and Characterization of its Interaction With PfAIP.
- Source :
-
Journal of molecular biology [J Mol Biol] 2020 Feb 14; Vol. 432 (4), pp. 878-896. Date of Electronic Publication: 2019 Dec 23. - Publication Year :
- 2020
-
Abstract
- Apicomplexan parasites contain rhoptries, which are specialized secretory organelles that coordinate host cell invasion. During the process of invasion, rhoptries secrete their contents to facilitate interaction with, and entry into, the host cell. Here, we report the crystal structure of the rhoptry protein Armadillo Repeats-Only (ARO) from the human malaria parasite, Plasmodium falciparum (PfARO). The structure of PfARO comprises five tandem Armadillo-like (ARM) repeats, with adjacent ARM repeats stacked in a head-to-tail orientation resulting in PfARO adopting an elongated curved shape. Interestingly, the concave face of PfARO contains two distinct patches of highly conserved residues that appear to play an important role in protein-protein interaction. We functionally characterized the P. falciparum homolog of ARO interacting protein (PfAIP) and demonstrate that it localizes to the rhoptries. We show that conditional mislocalization of PfAIP leads to deficient red blood cell invasion. Guided by the structure, we identified mutations of PfARO that lead to mislocalization of PfAIP. Using proximity-based biotinylation we probe into PfAIP interacting proteins.<br /> (Copyright © 2019. Published by Elsevier Ltd.)
- Subjects :
- Amino Acid Sequence
Animals
Armadillo Domain Proteins genetics
Armadillo Domain Proteins metabolism
Humans
Malaria physiopathology
Molecular Sequence Data
Mutagenesis genetics
Mutagenesis physiology
Mutation
Parasitemia parasitology
Phylogeny
Plasmodium falciparum genetics
Protein Transport genetics
Protein Transport physiology
Protozoan Proteins genetics
Plasmodium falciparum metabolism
Plasmodium falciparum pathogenicity
Protozoan Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8638
- Volume :
- 432
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 31877322
- Full Text :
- https://doi.org/10.1016/j.jmb.2019.12.024