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FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda.
- Source :
-
The Journal of veterinary medical science [J Vet Med Sci] 2020 Feb 18; Vol. 82 (2), pp. 188-192. Date of Electronic Publication: 2019 Dec 25. - Publication Year :
- 2020
-
Abstract
- East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings.
- Subjects :
- Animals
Cattle
Cattle Diseases blood
Cattle Diseases parasitology
DNA analysis
DNA isolation & purification
Dairying
Paper
Polymerase Chain Reaction economics
Polymerase Chain Reaction methods
Prevalence
Sodium Hydroxide
Specimen Handling instrumentation
Theileria parva genetics
Theileriasis blood
Theileriasis epidemiology
Uganda
Cattle Diseases diagnosis
Polymerase Chain Reaction veterinary
Theileria parva isolation & purification
Theileriasis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1347-7439
- Volume :
- 82
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of veterinary medical science
- Publication Type :
- Academic Journal
- Accession number :
- 31875599
- Full Text :
- https://doi.org/10.1292/jvms.19-0521