Lipid and poly (ethylene glycol)-conjugated bi-functionalized chlorine e6 micelles for NIR-light induced photodynamic therapy.

Authors :: Muddineti OS
Kiran Rompicharla SV
Kumari P
Bhatt H
Ghosh B
Biswas S
Source :: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2020 Mar; Vol. 29, pp. 101633. Date of Electronic Publication: 2019 Dec 20.
Publication Year :: 2020
Abstract: Background: To develop a photosensitizer, chlorin e6 (Ce6)-based amphiphilic polymer, DP-Ce6, where DOPE and PEG are conjugated to Ce6, which would self-assemble to form polymeric micelles (DP-Ce6-M) in aqueous environment.<br />Methods: DP-Ce6-M were characterized for particle size, zeta potential, and singlet oxygen ( <superscript>1</superscript> O <subscript>2</subscript> ) generation. Cellular internalization, phototoxicity were investigated against monolayer and 3D spheroids of human lung adenocarcinoma cells (A549).<br />Results and Conclusions: DP-Ce6-M formed stable micelles with particles size of 58.2 ± 1.6 nm. Solubility of Ce6 was improved. Photoactivity of DP-Ce6-M was sustained in regard to <superscript>1</superscript> O <subscript>2</subscript> generation compared to free Ce6. The DP-Ce6-M showed enhanced internalization and growth inhibition in monolayer and spheroidal cells. Overall, DP-Ce6-M demonstrated the potential for further exploration as PDT agent for cancer treatment.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)

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