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The conserved 3' UTR-derived small RNA NarS mediates mRNA crossregulation during nitrate respiration.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2020 Feb 28; Vol. 48 (4), pp. 2126-2143. - Publication Year :
- 2020
-
Abstract
- Small noncoding RNAs (sRNAs) from mRNA 3' UTRs seem to present a previously unrecognized layer of bacterial post-transcriptional control whereby mRNAs influence each other's expression, independently of transcriptional control. Studies in Escherichia coli and Salmonella enterica showed that such sRNAs are natural products of RNase E-mediated mRNA decay and associate with major RNA-binding proteins (RBPs) such as Hfq and ProQ. If so, there must be additional sRNAs from mRNAs that accumulate only under specific physiological conditions. We test this prediction by characterizing candidate NarS that represents the 3' UTR of nitrate transporter NarK whose gene is silent during standard aerobic growth. We find that NarS acts by Hfq-dependent base pairing to repress the synthesis of the nitrite transporter, NirC, resulting in mRNA cross-regulation of nitrate and nitrite transporter genes. Interestingly, the NarS-mediated repression selectively targets the nirC cistron of the long nirBDC-cysG operon, an observation that we rationalize as a mechanism to protect the bacterial cytoplasm from excessive nitrite toxicity during anaerobic respiration with abundant nitrate. Our successful functional assignment of a 3' UTR sRNA from a non-standard growth condition supports the notion that mRNA crossregulation is more pervasive than currently appreciated.<br /> (© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- 3' Untranslated Regions genetics
Endoribonucleases genetics
Escherichia coli genetics
Gene Expression Regulation, Bacterial
Nitrate Transporters
Nitrates metabolism
Operon genetics
RNA Processing, Post-Transcriptional genetics
RNA Stability genetics
RNA, Messenger genetics
RNA-Binding Proteins genetics
Respiration genetics
Salmonella enterica genetics
Anion Transport Proteins genetics
Escherichia coli Proteins genetics
Host Factor 1 Protein genetics
Methyltransferases genetics
RNA, Small Untranslated genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 48
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 31863581
- Full Text :
- https://doi.org/10.1093/nar/gkz1168