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Molecular Cloning and Expression of a Cryptochrome Gene CiCRY-DASH1 from the Antarctic microalga Chlamydomonas sp. ICE-L.
- Source :
-
Molecular biotechnology [Mol Biotechnol] 2020 Feb; Vol. 62 (2), pp. 91-103. - Publication Year :
- 2020
-
Abstract
- Cryptochromes (CRYs) are flavin-binding proteins that sense blue and near-ultraviolet light and participate in the photoreactions of organisms and the regulation of biological clocks. In this study, the complete open reading frame (ORF) of CiCRY-DASH1 (GenBank ID MK392361), encoding one kind of cryptochrome, was cloned from the Antarctic microalga Chlamydomonas sp. ICE-L. The quantitative real-time PCR study showed that the CiCRY-DASH1 had the highest expression at 5 °C and salinity of 32‰. The CiCRY-DASH1 was positively regulated by blue, yellow, or red light. Moreover, the CiCRY-DASH1 can positively respond to extreme polar day and night treatment and exhibit a certain circadian rhythm, which indicated that CiCRY-DASH1 participated in the circadian clock and its expression was regulated by circadian rhythms. And the CiCRY-DASH1 was more noticeably affected by ultraviolet-B radiation than ultraviolet-A radiation, indicating ultraviolet-B light does obvious damage to Antarctic microalgae.
- Subjects :
- Amino Acid Sequence
Antarctic Regions
Chlamydomonas chemistry
Chlamydomonas growth & development
Chlorophyll chemistry
Chlorophyll radiation effects
Circadian Rhythm
Cloning, Molecular
Computational Biology
Cryptochromes chemistry
Cryptochromes genetics
DNA, Complementary genetics
Gene Expression
Light
Microalgae metabolism
Open Reading Frames
Phylogeny
Salinity
Sequence Homology, Amino Acid
Temperature
Ultraviolet Rays
Chlamydomonas genetics
Cryptochromes metabolism
Cryptochromes radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1559-0305
- Volume :
- 62
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31863411
- Full Text :
- https://doi.org/10.1007/s12033-019-00225-y