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Roseomonas vastitatis sp. nov. isolated from Badain Jaran desert in China.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 Feb; Vol. 70 (2), pp. 1186-1191. - Publication Year :
- 2020
-
Abstract
- A Gram-stain-negative, non-motile, coccobacillus-shaped bacterium, designated CPCC 101021 <superscript>T</superscript> , was isolated from a sandy soil sample collected from Badain Jaran desert, China. Its 16S rRNA gene sequence was closely related to those of members of the genus Roseomonas, showing high similarities with Roseomonas hibiscisoli THG-N2.22 <superscript>T</superscript> (98.0 %), Roseomonas oryzae KCTC 42542 <superscript>T</superscript> (97.9 %), Roseomonas rhizosphaerae YW11 <superscript>T</superscript> (97.9 %) and Roseomonas suffusca S1 <superscript>T</superscript> (97.8 %). In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 101021 <superscript>T</superscript> formed a distinct subclade with R. oryzae KCTC 42542 <superscript>T</superscript> within the genus Roseomonas . Growth of the isolate occurred at 15-37 °C and pH 6.0-8.5, with optimal growth at 30 °C and pH 7.0. The major cellular fatty acids were C <subscript>18 : 1</subscript> ω 7c , summed feature 8 (C <subscript>16 : 1</subscript> ω 7 c/C <subscript>16 : 1</subscript> ω 6c ), summed feature 3 (C <subscript>16 : 1</subscript> ω 6 c/C <subscript>16 : 1</subscript> ω 7c ) and C <subscript>16 : 0</subscript> ω 6c . Q-10 was detected as the main component in the respiratory quinone system, with Q-9 as a minor component. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and an unidentified glycolipid were found in the polar lipid profile. The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity was 84.6 % when comparing the draft genome sequences of strain CPCC 101021 <superscript>T</superscript> with R. oryzae KCTC 42542 <superscript>T</superscript> . On the basis of genotypic, chemotaxonomic and phenotypic characteristics, strain CPCC 101021 <superscript>T</superscript> is proposed to represent a novel species of the genus Roseomonas with the name Roseomonas vastitatis sp. nov. Strain CPCC 101021 <superscript>T</superscript> (=J1A743 <superscript>T</superscript> =KCTC 62043 <superscript>T</superscript> ) is the type strain of the species.
- Subjects :
- Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial genetics
Fatty Acids chemistry
Glycolipids chemistry
Methylobacteriaceae isolation & purification
Nucleic Acid Hybridization
Phospholipids chemistry
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Ubiquinone analogs & derivatives
Ubiquinone chemistry
Desert Climate
Methylobacteriaceae classification
Phylogeny
Sand microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 70
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 31860433
- Full Text :
- https://doi.org/10.1099/ijsem.0.003898