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Recombinant Penicillium oxalicum 16 β-Glucosidase 1 Displays Comprehensive Inhibitory Resistance to Several Lignocellulose Pretreatment Products, Ethanol, and Salt.
- Source :
-
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2020 Jun; Vol. 191 (2), pp. 772-784. Date of Electronic Publication: 2019 Dec 20. - Publication Year :
- 2020
-
Abstract
- β-Glucosidase (BGL) is a rate-limiting enzyme of lignocellulose hydrolysis for second-generation bioethanol production, but its inhibition by lignocellulose pretreatment products, ethanol, and salt is apparent. Here, the recombinant Penicillium oxalicum 16 BGL 1 (rPO16BGL1) from Pichia pastoris GS115 kept complete activity at 0.2-1.4 mg/mL furan derivatives and phenolic compounds, 50 mg/mL sodium chloride (potassium chloride), or 100 mg/mL ethanol at 40 °C. rPO16BGL1 retained above 50% residual activity at 30 mg/mL organic acid sodium, and 60% residual activity at 40 °C with 300 mg/mL ethanol. Sodium chloride and potassium chloride had a complicated effect on rPO16BGL1, which resulted in activation or inhibition. The inhibition kinetics of the enzyme reaction demonstrated that organic acids and organic acid sodium were non-competitive inhibitors and that ethanol was a competitive inhibitor at < 1.5 mg/mL salicin. Moreover, substrate inhibition of the enzyme was found at > 2 mg/mL salicin, and the K <subscript>m</subscript> /K <subscript>I</subscript> and K <subscript>m</subscript> /K <subscript>SI</subscript> average values revealed that the inhibitory strength was ranked as salicin-organic acids > organic acids > salicin-organic acid sodium salt > organic acid sodium salt > salicin > salicin-KCl > salicin-NaCl > salicin-ethanol > ethanol.
- Subjects :
- Enzyme Activation drug effects
Enzyme Stability
Gene Expression Regulation, Fungal
Hydrolysis
Kinetics
Potassium Chloride
Saccharomycetales genetics
Sequence Analysis
Sodium Chloride pharmacology
beta-Glucosidase chemistry
Ethanol antagonists & inhibitors
Lignin antagonists & inhibitors
Penicillium genetics
Salts antagonists & inhibitors
beta-Glucosidase drug effects
beta-Glucosidase genetics
beta-Glucosidase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1559-0291
- Volume :
- 191
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Applied biochemistry and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31858406
- Full Text :
- https://doi.org/10.1007/s12010-019-03183-y