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Balanced Th1/Th2 immune response induced by MSP1a functional motif coupled to multiwalled carbon nanotubes as anti-anaplasmosis vaccine in murine model.
- Source :
-
Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2020 Feb; Vol. 24, pp. 102137. Date of Electronic Publication: 2019 Dec 16. - Publication Year :
- 2020
-
Abstract
- Anaplasmosis is one of the most prevalent tick-borne diseases of cattle caused by Anaplasma marginale. MSP1a surface protein has been shown to be involved in eliciting immunity to infected cattle. Carbon nanotubes (CNTs) has been increasingly highlighted due to their needle like structure, which contain multiple attachment sites for biomolecules and may interact with or cross biological membranes, increasing antigen availability to immune system. Here, we have successfully designed a nanocomplex of a synthetic peptide noncovalently attached to multiwalled CNT (MWCNT). Peptide comprising the core motif of the MSP1a was efficiently adsorb onto the nanoparticle surface. The MWCNT-Am1 nanocomplex exhibited high stability and good dispersibility and in vivo immunization showed high levels of IgG1 and IgG2a, followed by increased expression of pro-inflammatory and anti-inflammatory cytokines. This is a proof-of-concept of a nanovaccine that was able to generate a strong immune response compared to the common antigen-adjuvant vaccine without the nanoparticles.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Anaplasma immunology
Anaplasma pathogenicity
Anaplasmosis prevention & control
Animals
Antigens, Bacterial chemistry
Cell Survival drug effects
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Mice
Mice, Inbred BALB C
Polymerase Chain Reaction
Anaplasmosis immunology
Antigens, Bacterial immunology
Bacterial Outer Membrane Proteins chemistry
Bacterial Outer Membrane Proteins immunology
Nanotubes, Carbon chemistry
Th1 Cells immunology
Th2 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1549-9642
- Volume :
- 24
- Database :
- MEDLINE
- Journal :
- Nanomedicine : nanotechnology, biology, and medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31857182
- Full Text :
- https://doi.org/10.1016/j.nano.2019.102137