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LncRNA RMST-mediated miR-107 transcription promotes OGD-induced neuronal apoptosis via interacting with hnRNPK.
- Source :
-
Neurochemistry international [Neurochem Int] 2020 Feb; Vol. 133, pp. 104644. Date of Electronic Publication: 2019 Dec 15. - Publication Year :
- 2020
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Abstract
- The long noncoding RNA (lncRNA) rhabdomyosarcoma 2-associated transcript (RMST) silencing has been demonstrated to protect against ischemic brain injury in vivo and neuron injury in vitro. However, its underlying mechanisms in the progression of ischemic stroke have not been well explored. The expression of RMST in oxygen-glucose deprivation (OGD)-treated HT-22 hippocampal neuron cell line was examined using quantitative Real-Time PCR (qRT-PCR). CCK-8 cell viability and apoptotic cell detection using Annexin V-FITC and PI staining coupled with flow cytometry were performed to determine the pro-apoptotic role of RMST in HT-22 hippocampal neuron cell line. Furthermore, RNA pull-down, RNA immunoprecipitation (RIP), coimmunoprecipitation (Co-IP), chromatin immunoprecipitation (ChIP) and dual-Luciferase reporter assays were performed to determine the mechanism of RMST in OGD-induced HT-22 cell apoptosis. In the results, RMST was highly expressed in OGD-treated HT-22 cells. Altered RMST expression led to marked changes in HT-22 cell proliferation and apoptosis. Mechanistically, RMST indirectly activated p53/miR-107 signaling pathway via interacting with heterogeneous nuclear ribonucleoprotein K (hnRNPK) and fulfilled its pro-apoptotic function in HT-22 cells. In conclusion, our data indicated that the RMST/hnRNPK/p53/miR-107/Bcl2l2 axis plays an important role in regulating neuronal apoptosis.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Proliferation physiology
Glucose metabolism
Heterogeneous-Nuclear Ribonucleoprotein K metabolism
Mice
Neurons metabolism
Oxygen metabolism
Signal Transduction genetics
Stroke metabolism
Apoptosis genetics
Heterogeneous-Nuclear Ribonucleoprotein K genetics
MicroRNAs genetics
RNA, Long Noncoding genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9754
- Volume :
- 133
- Database :
- MEDLINE
- Journal :
- Neurochemistry international
- Publication Type :
- Academic Journal
- Accession number :
- 31852624
- Full Text :
- https://doi.org/10.1016/j.neuint.2019.104644