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miR-4324 functions as a tumor suppressor in colorectal cancer by targeting HOXB2.
- Source :
-
The Journal of international medical research [J Int Med Res] 2020 Mar; Vol. 48 (3), pp. 300060519883731. Date of Electronic Publication: 2019 Dec 18. - Publication Year :
- 2020
-
Abstract
- Objective: MicroRNAs (miRNAs) are reported to have crucial roles in human cancers; however, their role in colorectal cancer (CRC) remains largely unknown.<br />Methods: In this study, we analyzed the expression of miR-4324 in CRC cell lines using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We also examined miR-4324 expression in CRC tumor tissues using a miRNA expression dataset obtained from the Gene Expression Omnibus. We validated the connection between miR-4324 and homeobox B2 (HOXB2) using a luciferase activity reporter assay and western blotting. The effects of miR-4324 and HOXB2 on CRC cell malignant behaviors in vitro were further investigated.<br />Results: miR-4324 expression was significantly decreased in both CRC tumor tissues and cell lines. Overexpression of miR-4324 suppressed CRC cell proliferation, migration, and invasion. In contrast, overexpression of HOXB2 promoted CRC malignant cell behaviors. Furthermore, we validated HOXB2 as a direct target of miR-4324.<br />Conclusions: miR-4324 expression was decreased in CRC. miR-4324 regulates CRC cell proliferation, migration, and invasion by targeting HOXB2.
Details
- Language :
- English
- ISSN :
- 1473-2300
- Volume :
- 48
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of international medical research
- Publication Type :
- Academic Journal
- Accession number :
- 31852342
- Full Text :
- https://doi.org/10.1177/0300060519883731