Agromyces protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis .

An actinobacterial strain, designated FW100M-8 ^T , was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. Cells were Gram-stain-positive, microaerophilic to aerobic, non-spore forming and non-motile. It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %). According to the 16S rRNA gene analysis, strain FW100M-8 ^T shared the highest sequence similarity with Agromyces mediolanus DSM 20152 ^T (98.4 %), Agromyces ulmi XIL01 ^T (98.3 %), Agromyces indicus NIO-1018 ^T (98.3 %), Agromyces soli MJ21 ^T (98.3 %), and Agromyces arachidis AK-1 ^T (97.9 %). Phylogenetic trees showed that strain FW100M-8 ^T fell into the lineage of the genus Agromyces . The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The menaquinones of strain FW100M-8 ^T were MK-12 (46 %), MK-11 (36 %), MK-10 (14 %) and MK-13 (4 %). The major fatty acids were anteiso-C _15 : 0 , anteiso-C _17 : 0 and iso-C _16 : 0 . The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A ₂ bu. The G+C content of the genomic DNA is 70.5 mol%. On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8 ^T represents a novel species of the genus Agromyces , for which the name Agromyces protaetiae sp. nov. is proposed with strain FW100M-8 ^T (=KACC 19308 ^T =NBRC 113048 ^T ) as the type strain.