Back to Search
Start Over
Multidrug Adaptive Resistance of Pseudomonas aeruginosa Swarming Cells.
- Source :
-
Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2020 Feb 21; Vol. 64 (3). Date of Electronic Publication: 2020 Feb 21 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Swarming surface motility is a complex adaptation leading to multidrug antibiotic resistance and virulence factor production in Pseudomonas aeruginosa Here, we expanded previous studies to demonstrate that under swarming conditions, P. aeruginosa PA14 is more resistant to multiple antibiotics, including aminoglycosides, β-lactams, chloramphenicol, ciprofloxacin, tetracycline, trimethoprim, and macrolides, than swimming cells, but is not more resistant to polymyxin B. We investigated the mechanism(s) of swarming-mediated antibiotic resistance by examining the transcriptomes of swarming cells and swarming cells treated with tobramycin by transcriptomics (RNA-Seq) and reverse transcriptase quantitative PCR (qRT-PCR). RNA-Seq of swarming cells (versus swimming) revealed 1,581 dysregulated genes, including 104 transcriptional regulators, two-component systems, and sigma factors, numerous upregulated virulence and iron acquisition factors, and downregulated ribosomal genes. Strain PA14 mutants in resistome genes that were dysregulated under swarming conditions were tested for their ability to swarm in the presence of tobramycin. In total, 41 mutants in genes dysregulated under swarming conditions were shown to be more resistant to tobramycin under swarming conditions, indicating that swarming-mediated tobramycin resistance was multideterminant. Focusing on two genes downregulated under swarming conditions, both prtN and wbpW mutants were more resistant to tobramycin, while the prtN mutant was additionally resistant to trimethoprim under swarming conditions; complementation of these mutants restored susceptibility. RNA-Seq of swarming cells treated with subinhibitory concentrations of tobramycin revealed the upregulation of the multidrug efflux pump MexXY and downregulation of virulence factors.<br /> (Copyright © 2020 American Society for Microbiology.)
- Subjects :
- Aminoglycosides pharmacology
Chloramphenicol pharmacology
Ciprofloxacin pharmacology
Drug Resistance, Microbial genetics
Gene Expression Regulation, Bacterial
Macrolides pharmacology
Mutation
Pseudomonas aeruginosa genetics
Pseudomonas aeruginosa physiology
RNA-Seq
Reverse Transcriptase Polymerase Chain Reaction
Tetracycline pharmacology
Tobramycin pharmacology
Trimethoprim pharmacology
Pseudomonas aeruginosa drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1098-6596
- Volume :
- 64
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Antimicrobial agents and chemotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 31844008
- Full Text :
- https://doi.org/10.1128/AAC.01999-19