B lymphocytopenia and Bregs in a not-to-die murine sepsis model.

Authors :: Umakoshi K
Choudhury ME
Nishioka R
Matsumoto H
Abe N
Nishikawa Y
Kikuchi S
Takeba J
Yano H
Yorozuya T
Sato N
Aibiki M
Tanaka J
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Feb 26; Vol. 523 (1), pp. 202-207. Date of Electronic Publication: 2019 Dec 14.
Publication Year :: 2020
Abstract: Sepsis is a leading cause of mortality in intensive care units due to multi-organ failure caused by dysregulated immune reactions. In this study, kinetic changes in the immune system were analyzed for 72 h in cecal ligation and puncture (CLP)-induced septic mice while preventing animal death by keeping body temperature. Increase of myeloid cells and decrease of B cells in circulation at 6 h after CLP were markedly observed. At the same time point, interleukin (IL)-10 expressing CD5 <superscript>+</superscript> regulatory B cells (Bregs) appeared. IL-10 and programmed death-ligand 1 (PD-L1) mRNA as well as IL-1β, IL-6 and interferon γ (IFNγ) mRNA was increased in the spleen at 6 h. A gradual decrease in Bcl-2 and abrupt increase of Bim expression in the spleen at the late phase were also found. These results showed that B lymphocytopenia with the appearance of Bregs is the earliest event, likely leading to immunoparalysis in sepsis.<br />Competing Interests: Declaration of competing interest Nothing to declare.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)