Back to Search
Start Over
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
- Source :
-
Cell reports [Cell Rep] 2019 Dec 10; Vol. 29 (11), pp. 3605-3619.e10. - Publication Year :
- 2019
-
Abstract
- Ribosome assembly occurs mainly in the nucleolus, yet recent studies have revealed robust enrichment and translation of mRNAs encoding many ribosomal proteins (RPs) in axons, far away from neuronal cell bodies. Here, we report a physical and functional interaction between locally synthesized RPs and ribosomes in the axon. We show that axonal RP translation is regulated through a sequence motif, CUIC, that forms an RNA-loop structure in the region immediately upstream of the initiation codon. Using imaging and subcellular proteomics techniques, we show that RPs synthesized in axons join axonal ribosomes in a nucleolus-independent fashion. Inhibition of axonal CUIC-regulated RP translation decreases local translation activity and reduces axon branching in the developing brain, revealing the physiological relevance of axonal RP synthesis in vivo. These results suggest that axonal translation supplies cytoplasmic RPs to maintain/modify local ribosomal function far from the nucleolus in neurons.<br /> (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Axons ultrastructure
Brain cytology
Brain growth & development
Brain metabolism
Cells, Cultured
RNA, Messenger genetics
RNA, Messenger metabolism
Regulatory Sequences, Ribonucleic Acid
Ribosomal Proteins metabolism
Ribosomes genetics
Xenopus laevis
Axons metabolism
Neurogenesis
Ribosomal Proteins genetics
Ribosomes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 29
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 31825839
- Full Text :
- https://doi.org/10.1016/j.celrep.2019.11.025