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Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
- Source :
-
Inflammatory bowel diseases [Inflamm Bowel Dis] 2020 Apr 11; Vol. 26 (5), pp. 697-708. - Publication Year :
- 2020
-
Abstract
- Background: The gut microbiota is a key element to support host homeostasis and the development of the immune system. The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes. In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes. In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.<br />Results: We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes. The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice. In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice. To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis. Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.<br />Conclusions: Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.<br /> (© 2019 Crohn’s & Colitis Foundation. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Animals
Colitis chemically induced
Colitis immunology
Colitis microbiology
Dextran Sulfate
Disease Models, Animal
Feces chemistry
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Gastrointestinal Microbiome immunology
Homeostasis immunology
Immunoglobulin A analysis
Intestines immunology
Natural Killer T-Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1536-4844
- Volume :
- 26
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Inflammatory bowel diseases
- Publication Type :
- Academic Journal
- Accession number :
- 31819985
- Full Text :
- https://doi.org/10.1093/ibd/izz300