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Serological evidence of DENV, JEV, and ZIKV among the indigenous people (Orang Asli) of Peninsular Malaysia.
- Source :
-
Journal of medical virology [J Med Virol] 2020 Aug; Vol. 92 (8), pp. 956-962. Date of Electronic Publication: 2019 Dec 18. - Publication Year :
- 2020
-
Abstract
- Dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV) are mosquito-borne flavivirus of medical importance in tropical countries such as Malaysia. However, much remains unknown regarding their prevalence among the underserved indigenous people (Orang Asli) living in communities in the forest fringe areas of Peninsular Malaysia. Information on the prevalence of diseases is necessary to elevate the effectiveness of disease control and preventive measures. This study aimed to determine the seroprevalence of the three major flaviviruses among the Orang Asli and investigate the association between demographic factors and seropositivities. Sampling activities were conducted in the Orang Asli villages to obtain serum samples and demographic data from consenting volunteers. The presence of DENV, JEV, and ZIKV immunoglobulin G (IgG) antibodies in the sera were examined using commercial enzyme-linked immunosorbent assay kits. A focus reduction neutralization assay was performed to measure virus-specific neutralizing antibodies. A total of 872 serum samples were obtained from the Orang Asli volunteers. Serological assay results revealed that DENV IgG, JEV IgG, and ZIKV IgG seropositivities among the Orang Asli were at 4.9%, 48.4%, and 13.2%, respectively. Neutralizing antibodies (FRNT50 ≥ 1:40) against JEV and ZIKV were found in 86.7% and 100.0%, respectively, out of the samples tested. Positive serology to all three viruses corresponded significantly to the age of the volunteers with increasing seropositivity in older volunteers. Findings from the study suggest that Orang Asli are at significant risk of contracting JEV and ZIKV infections despite the lack of active transmission of the viruses in the country.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Adult
Antibodies, Neutralizing blood
Cross Reactions
Dengue Virus immunology
Encephalitis Virus, Japanese immunology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin G blood
Malaysia epidemiology
Male
Seroepidemiologic Studies
Young Adult
Zika Virus immunology
Antibodies, Viral blood
Dengue epidemiology
Encephalitis, Japanese epidemiology
Flavivirus immunology
Indigenous Peoples
Zika Virus Infection epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-9071
- Volume :
- 92
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of medical virology
- Publication Type :
- Academic Journal
- Accession number :
- 31814135
- Full Text :
- https://doi.org/10.1002/jmv.25649