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Fluorescent gold nanocluster-based sensor for detection of alkaline phosphatase in human osteosarcoma cells.
- Source :
-
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2020 Mar 15; Vol. 229, pp. 117875. Date of Electronic Publication: 2019 Nov 30. - Publication Year :
- 2020
-
Abstract
- Gold nanoclusters (AuNCs) have attracted much attention as signal transducers in photoluminescence chemical/biological sensors. Herein, we employ bovine serum albumin/3-mercaptopropionic acid co-modified AuNCs as a fluorescence probe, Fe <superscript>3+</superscript> as a quencher, and pyrophosphate as an alkaline phosphatase (ALP) substrate and Fe <superscript>3+</superscript> chelator to design a novel biosensor for ALP detection, achieving a detection linear range of 0.8-16 U/L and a detection limit of 0.78 U/L. The developed method is successfully applied to the detection of ALP in human osteosarcoma cells and is shown to be suited for ALP inhibitor screening.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Bone Neoplasms pathology
Cell Line, Tumor
Humans
Osteosarcoma pathology
Alkaline Phosphatase metabolism
Bone Neoplasms enzymology
Fluorescent Dyes chemistry
Fluorescent Dyes pharmacology
Gold chemistry
Gold pharmacology
Metal Nanoparticles chemistry
Neoplasm Proteins metabolism
Osteosarcoma enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3557
- Volume :
- 229
- Database :
- MEDLINE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 31813715
- Full Text :
- https://doi.org/10.1016/j.saa.2019.117875