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A fast-dissolving microneedle array loaded with chitosan nanoparticles to evoke systemic immune responses in mice.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2020 Jan 14; Vol. 8 (2), pp. 216-225. Date of Electronic Publication: 2019 Dec 05. - Publication Year :
- 2020
-
Abstract
- Microneedle (MN) arrays offer an alternative approach to hypodermic injection via syringe needles. In this work, polyvinylpyrrolidone (PVP)-based fast dissolving MN arrays were developed in which the needle tips were loaded with chitosan nanoparticles (NPs) for coencapsulation of a model antigen, ovalbumin (OVA), and an adjuvant, CpG oligodeoxynucleotides (CpG). After insertion into the skin, these MN arrays fully dissolved within 3 min to release antigen and adjuvant co-loaded NPs rapidly in the epidermal layer. Positively charged chitosan was proven to be an excellent carrier for negatively charged OVA and CpG, which formed nanocomplexes via simple electrostatic interactions and greatly enhanced the uptake efficiency of OVA in DC2.4 dendritic cells. Vaccination studies in mice further demonstrated that chitosan NPs effectively accumulated in peripheral lymph nodes, thus inducing greatly enhanced immune responses compared to those of free OVA. The antibody dose-response curve further demonstrated that MN immunization achieved comparable levels of immune responses as compared to conventional subcutaneous injections in a more convenient and less invasive way. Overall, a PVP-based fast dissolving MN array with chitosan NPs represents a promising and robust platform system for efficient transcutaneous vaccine delivery.
- Subjects :
- Adjuvants, Immunologic administration & dosage
Adjuvants, Immunologic pharmacology
Administration, Cutaneous
Animals
Antigens administration & dosage
Antigens pharmacology
Chitosan chemistry
Female
Mice
Nanoparticles chemistry
Oligodeoxyribonucleotides administration & dosage
Ovalbumin administration & dosage
Drug Delivery Systems methods
Immunization methods
Injections, Intradermal methods
Microinjections methods
Oligodeoxyribonucleotides pharmacology
Ovalbumin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 8
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 31803892
- Full Text :
- https://doi.org/10.1039/c9tb02061f