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[Protective effect of Lactobacillus reuteri against oxidative stress in neonatal mice with necrotizing enterocolitis].
- Source :
-
Nan fang yi ke da xue xue bao = Journal of Southern Medical University [Nan Fang Yi Ke Da Xue Xue Bao] 2019 Oct 30; Vol. 39 (10), pp. 1221-1226. - Publication Year :
- 2019
-
Abstract
- Objective: To investigate the protective effect of L. reuteri DSM17938 strain against oxidative stress in a neonatal mouse model of necrotizing enterocolitis (NEC) and explore the possible mechanism.<br />Methods: Ninety-six 10-day-old neonatal C57BL/6J mice were equally randomized into control group, NEC group, and NEC+ L. reuteri group. The pathological changes of the ileocecal intestinal tissue were evaluated with HE staining and double-blind pathological scoring. The mRNA and protein expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the intestinal tissues were detected using quantitative real-time PCR and ELISA, respectively. Colorimetric assays were used to determine the activity of superoxide dismutase (SOD) and its inhibition rate, malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG), and GSSG/ GSH ratio.<br />Results: Compared with those in the control group, the neonatal mice in NEC group showed significant weight loss ( P < 0.05), obvious intestinal injury, increased pathological scores ( P < 0.05), increased expressions of TNF-α and IL-1β mRNA and proteins ( P < 0.05), decreased SOD activity and inhibition rate, decreased GSH, and significantly increased MDA, GSSG, and GSSG/GSH ratios ( P < 0.05). Treatment with L. reuteri obviously decreased the pathological scores, expressions of TNF-α and IL-1β ( P < 0.05), MDA, GSSG, and GSSG/GSH ratio ( P < 0.05), and significantly increased SOD activity, its inhibition rate, and GSH level in the mice with NEC, but the survival rate was not significantly different between NEC and L. reuteri -treated groups ( P > 0.05).<br />Conclusions: L. reuteri DSM17938 can offer protection against NEC in mice by reducing oxidative stress and increasing antioxidant capacity of the intestinal tissue to suppress intestinal inflammations.
Details
- Language :
- Chinese
- ISSN :
- 1673-4254
- Volume :
- 39
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University
- Publication Type :
- Academic Journal
- Accession number :
- 31801706
- Full Text :
- https://doi.org/10.12122/j.issn.1673-4254.2019.10.14