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Revealing the mechanism of DNA passing through graphene and boron nitride nanopores.
- Source :
-
Nanoscale [Nanoscale] 2019 Dec 28; Vol. 11 (48), pp. 23438-23448. Date of Electronic Publication: 2019 Dec 04. - Publication Year :
- 2019
-
Abstract
- Nanopores on 2D materials have great potential for DNA sequencing, which is attributed to their high sequencing speed and reduced cost. However, identifying DNA bases at such a high speed with nanometer precision has remained a big challenge. Here, we implemented theoretical calculations to show the translocation of single-stranded DNA (ssDNA) through solid-state nanopores on a 2D hexagonal boron nitride (h-BN) and graphene sheet. A base-specific ssDNA sequencing technique was devised, based on the individual differences in the ion current responses for the (polyA) <subscript>16</subscript> , (polyG) <subscript>16</subscript> , (polyC) <subscript>16</subscript> , and (polyT) <subscript>16</subscript> bases of ssDNA. Our sequential procedure for sequencing is built on a comparative approach between the current signals obtained from the nanopores to achieve base-specific detection. Our results indicate that at higher voltages (1.0, 1.2, 1.4, 1.6, 1.8 and 2.0 V nm <superscript>-1</superscript> ), DNA translocation is tracked though the 1.5 and 2.0 nm nanopores, and at the 1.5 nm pore size, folded ssDNA close to the nanopore accounts for 93% and 81% of events for graphene and h-BN. Our calculations indicate charge transfer from the graphene to ssDNA, while the reverse happens in the case of the h-BN membrane. These results provide critical insights into our understanding of single molecule sequencing through solid-state nanopore research.
Details
- Language :
- English
- ISSN :
- 2040-3372
- Volume :
- 11
- Issue :
- 48
- Database :
- MEDLINE
- Journal :
- Nanoscale
- Publication Type :
- Academic Journal
- Accession number :
- 31799536
- Full Text :
- https://doi.org/10.1039/c9nr07651d