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Identification of Angiotensin I-Converting Enzyme-Inhibitory and Anticoagulant Peptides from Enzymatic Hydrolysates of Chicken Combs and Wattles.
- Source :
-
Journal of medicinal food [J Med Food] 2019 Dec; Vol. 22 (12), pp. 1294-1300. Date of Electronic Publication: 2019 Dec 02. - Publication Year :
- 2019
-
Abstract
- Peptides from protein hydrolysate of a mixture of chicken combs and wattles (CCWs) were obtained through enzymatic hydrolysis, and their anticoagulant and inhibitory effects on angiotensin I-converting enzyme (ACE) were investigated. The protein hydrolysate exhibited anticoagulant capacity by the intrinsic pathway (activated partial thromboplastin time) and potent ACE-inhibitory activity. The peptides were sequenced by LC-MS to identify those with higher inhibitory potential. From the pool of sequenced peptides, the following three peptides were selected and synthesized based on their low molecular weight and the presence of amino acids with ACE-inhibitory potential at the C-terminus: peptide I (APGLPGPR), peptide II (Piro-GPPGPT), and peptide III (FPGPPGP). Peptide III (FPGPPGP) showed the highest ACE-inhibitory capacity among the peptides selected. In conclusion, a peptide (FPGPPGP) of unknown sequence was identified as having potent ACE-inhibitory capacity. This peptide originated from unconventional hydrolysates from poultry slaughter waste, including combs and wattles.
- Subjects :
- Amino Acid Sequence
Angiotensin-Converting Enzyme Inhibitors isolation & purification
Animals
Chickens
Chromatography, Liquid
Humans
Hydrophobic and Hydrophilic Interactions
Molecular Weight
Peptides chemistry
Peptides isolation & purification
Protein Hydrolysates
Receptors for Activated C Kinase chemistry
Receptors for Activated C Kinase pharmacology
Thromboplastin
Angiotensin-Converting Enzyme Inhibitors pharmacology
Anticoagulants pharmacology
Comb and Wattles chemistry
Peptides pharmacology
Peptidyl-Dipeptidase A drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7600
- Volume :
- 22
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of medicinal food
- Publication Type :
- Academic Journal
- Accession number :
- 31794688
- Full Text :
- https://doi.org/10.1089/jmf.2019.0066