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Heterologous prime-boost vaccination against tuberculosis with recombinant Sendai virus and DNA vaccines.
- Source :
-
Journal of molecular medicine (Berlin, Germany) [J Mol Med (Berl)] 2019 Dec; Vol. 97 (12), pp. 1685-1694. Date of Electronic Publication: 2019 Nov 30. - Publication Year :
- 2019
-
Abstract
- In an earlier study, a novel Sendai virus-vectored anti-tuberculosis vaccine encoding Ag85A and Ag85B (SeV85AB) was constructed and shown to elicit antigen-specific T cell responses and protection against Mycobacterium tuberculosis (Mtb) infection in a murine model. In this study, we evaluate whether the immune responses induced by this novel vaccine might be elevated by a recombinant DNA vaccine expressing the same antigen in a heterologous prime-boost vaccination strategy. The results showed that both SeV85AB prime-DNA boost (SeV85AB-DNA) and DNA prime-SeV85AB boost (DNA-SeV85AB) vaccination strategies significantly enhanced the antigen-specific T cell responses induced by the separate vaccines. The SeV85AB-DNA immunization regimen induced higher levels of recall T cell responses after Mtb infection and conferred better immune protection compared with DNA-SeV85AB or a single immunization. Collectively, our study lends strong evidence that a DNA vaccine boost might be included in a novel SeV85AB immunization strategy designed to enhance the immune protection against Mtb. KEY MESSAGES: A heterologous prime-boost regimen with a novel recombinant SeV85AB and a DNA vaccine increase the T cell responses above those from a single vaccine. The heterologous prime-boost regimen provided protection against Mtb infection. The DNA vaccine might be included in a novel SeV85AB immunization strategy designed to enhance the immune protection against Mtb.
- Subjects :
- Acyltransferases genetics
Animals
Antigens, Bacterial genetics
Bacterial Proteins genetics
CD8-Positive T-Lymphocytes immunology
Female
Genetic Vectors
Immunization, Secondary
Interferon-gamma metabolism
Interleukin-2 metabolism
Mice
Mice, Inbred BALB C
Mycobacterium tuberculosis genetics
Sendai virus genetics
Tuberculosis prevention & control
Tuberculosis Vaccines immunology
Tumor Necrosis Factor-alpha metabolism
Vaccination
Acyltransferases immunology
Antigens, Bacterial immunology
Bacterial Proteins immunology
Mycobacterium tuberculosis immunology
Tuberculosis immunology
Tuberculosis Vaccines genetics
Vaccines, DNA immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1440
- Volume :
- 97
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of molecular medicine (Berlin, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 31786669
- Full Text :
- https://doi.org/10.1007/s00109-019-01844-3