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Core Components of the Nuclear Pore Bind Distinct States of Chromatin and Contribute to Polycomb Repression.
- Source :
-
Molecular cell [Mol Cell] 2020 Jan 02; Vol. 77 (1), pp. 67-81.e7. Date of Electronic Publication: 2019 Nov 26. - Publication Year :
- 2020
-
Abstract
- Interactions between the genome and the nuclear pore complex (NPC) have been implicated in multiple gene regulatory processes, but the underlying logic of these interactions remains poorly defined. Here, we report high-resolution chromatin binding maps of two core components of the NPC, Nup107 and Nup93, in Drosophila cells. Our investigation uncovered differential binding of these NPC subunits, where Nup107 preferentially targets active genes while Nup93 associates primarily with Polycomb-silenced regions. Comparison to Lamin-associated domains (LADs) revealed that NPC binding sites can be found within LADs, demonstrating a linear binding of the genome along the nuclear envelope. Importantly, we identified a functional role of Nup93 in silencing of Polycomb target genes and in spatial folding of Polycomb domains. Our findings lend to a model where different nuclear pores bind different types of chromatin via interactions with specific NPC sub-complexes, and a subset of Polycomb domains is stabilized by interactions with Nup93.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Aquaporins metabolism
Binding Sites physiology
Cell Line
Drosophila metabolism
Drosophila Proteins metabolism
Female
Gene Expression Regulation physiology
Genome physiology
Male
Nuclear Envelope metabolism
Nuclear Pore Complex Proteins metabolism
Chromatin metabolism
Nuclear Pore metabolism
Polycomb-Group Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4164
- Volume :
- 77
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular cell
- Publication Type :
- Academic Journal
- Accession number :
- 31784359
- Full Text :
- https://doi.org/10.1016/j.molcel.2019.10.017