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Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host.
- Source :
-
Genome biology [Genome Biol] 2019 Nov 25; Vol. 20 (1), pp. 252. Date of Electronic Publication: 2019 Nov 25. - Publication Year :
- 2019
-
Abstract
- Background: Recent metagenomic analyses have revealed dysbiosis of the gut microbiota of ulcerative colitis (UC) patients. However, the impacts of this dysbiosis are not fully understood, particularly at the strain level.<br />Results: We perform whole-genome shotgun sequencing of fecal DNA extracts from 13 healthy donors and 16 UC and 8 Crohn's disease (CD) patients. The microbiota of UC and CD patients is taxonomically and functionally divergent from that of healthy donors, with E. faecium being the most differentially abundant species between the two microbial communities. Transplantation of feces from UC or CD patients into Il10 <superscript>-/-</superscript> mice promotes pathological inflammation and cytokine expression in the mouse colon, although distinct cytokine expression profiles are observed between UC and CD. Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression. Inflammatory E. faecium strains, including ATCC 19434 and a UC-derived strain, cluster separately from commercially available probiotic strains based on whole-genome shotgun sequencing analysis. The presence of E. faecium in fecal samples is associated with large disease extent and the need for multiple medications in UC patients.<br />Conclusions: E. faecium strains derived from UC patients display an inflammatory genotype that causes colitis.
- Subjects :
- Animals
Case-Control Studies
Colitis etiology
Colitis pathology
Colitis, Ulcerative drug therapy
Colon pathology
Crohn Disease microbiology
Disease Models, Animal
Drug Therapy, Combination
Enterococcus faecalis genetics
Fecal Microbiota Transplantation
Feces microbiology
Female
Humans
Interleukin-10 genetics
Male
Mice
Mice, Inbred C57BL
Colitis, Ulcerative microbiology
Enterococcus faecalis pathogenicity
Gastrointestinal Microbiome
Metagenome
Subjects
Details
- Language :
- English
- ISSN :
- 1474-760X
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genome biology
- Publication Type :
- Academic Journal
- Accession number :
- 31767028
- Full Text :
- https://doi.org/10.1186/s13059-019-1879-9