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Dissecting the Regulatory Network of Leaf Premature Senescence in Maize ( Zea mays L.) Using Transcriptome Analysis of ZmELS5 Mutant.
- Source :
-
Genes [Genes (Basel)] 2019 Nov 19; Vol. 10 (11). Date of Electronic Publication: 2019 Nov 19. - Publication Year :
- 2019
-
Abstract
- Leaf premature senescence largely determines maize ( Zea mays L.) grain yield and quality. A natural recessive premature-senescence mutant was selected from the breeding population, and near-isogenic lines were constructed using Jing24 as the recurrent parent. In the near-isogenic lines, the dominant homozygous material was wild-type (WT), and the recessive material of early leaf senescence was the premature-senescence-type ZmELS5 . To identify major genes and regulatory mechanisms involved in leaf senescence, a transcriptome analysis of the ZmELS5 and WT near-isogenic lines (NILs) was performed. A total of 8,796 differentially expressed transcripts were identified between ZmELS5 and WT, including 3,811 up-regulated and 4,985 down-regulated transcripts. By combining gene ontology, Kyoto Encyclopedia of Genes and Genomes, gene set, and transcription factor enrichment analyses, key differentially expressed genes were screened. The senescence regulatory network was predicted based on these key differentially expressed genes, which indicated that the senescence process is mainly regulated by bHLH, WRKY, and AP2/EREBP family transcription factors, leading to the accumulations of jasmonic acid and ethylene. This causes stress responses and reductions in the chlorophyll a/b-binding protein activity level. Then, decreased ATP synthase activity leads to increased photosystem II photodamage, ultimately leading to leaf senescence.<br />Competing Interests: Authors declare no conflict of interest.
- Subjects :
- ATP Synthetase Complexes genetics
ATP Synthetase Complexes metabolism
Albinism, Oculocutaneous
Chlorophyll A
Cyclopentanes metabolism
Ethylenes metabolism
Gene Expression Profiling
Genes, Plant genetics
Oxylipins metabolism
Photosystem II Protein Complex genetics
Photosystem II Protein Complex metabolism
Plant Growth Regulators metabolism
Plant Leaves physiology
Plant Proteins metabolism
Plants, Genetically Modified
Transcription Factors genetics
Transcription Factors metabolism
Cellular Senescence genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Gene Regulatory Networks
Plant Proteins genetics
Zea mays physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 10
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 31752425
- Full Text :
- https://doi.org/10.3390/genes10110944