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Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp. reveals the basis for its salt tolerance.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2020 Mar 15; Vol. 147, pp. 1309-1317. Date of Electronic Publication: 2019 Nov 18. - Publication Year :
- 2020
-
Abstract
- Marine macroalgae have gained considerable attention as renewable biomass sources. Ulvan is a water-soluble anionic polysaccharide, and its depolymerization into fermentable monosaccharides has great potential for the production of bioethanol or high-value food additives. Ulvan lyase from Alteromonas sp. (AsPL) utilizes a β-elimination mechanism to cleave the glycosidic bond between rhamnose 3-sulfate and glucuronic acid, forming an unsaturated uronic acid at the non-reducing end. AsPL was active in the temperature range of 30-50 °C and pH values ranging from 7.5 to 9.5. Furthermore, AsPL was found to be halophilic, showing high activity and stability in the presence of up to 2.5 M NaCl. The apparent K <subscript>m</subscript> and k <subscript>cat</subscript> values of AsPL are 3.19 ± 0.37 mg mL <superscript>-1</superscript> and 4.19 ± 0.21 s <superscript>-1</superscript> , respectively. Crystal structure analysis revealed that AsPL adopts a β-propeller fold with four anti-parallel β-strands in each of the seven propeller blades. The acid residues at the protein surface and two Ca <superscript>2+</superscript> coordination sites contribute to its salt tolerance. The research on ulvan lyase has potential commercial value in the utilization of algal resources for biofuel production to relieve the environmental burden of petrochemicals.<br />Competing Interests: Declaration of Competing Interest The authors declare no competing financial interests.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Binding Sites
Biofuels
Calcium chemistry
Catalysis
Chromatography, Liquid
Circular Dichroism
Crystallography, X-Ray
Disaccharides chemistry
Glycosides chemistry
Hydrogen-Ion Concentration
Kinetics
Oligosaccharides chemistry
Protein Structure, Secondary
Seaweed
Temperature
Alteromonas enzymology
Glucuronic Acid chemistry
Polysaccharide-Lyases chemistry
Rhamnose chemistry
Salt Tolerance
Sulfates chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 147
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 31751708
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2019.10.095