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Serum IgE and IgG responses to dietary antigens in dogs with and without cutaneous adverse food reactions.
- Source :
-
Veterinary dermatology [Vet Dermatol] 2020 Apr; Vol. 31 (2), pp. 116-127. Date of Electronic Publication: 2019 Nov 20. - Publication Year :
- 2020
-
Abstract
- Background: It is suspected that many canine cutaneous adverse food reactions (CAFR) are true immunological hypersensitivities; however, few specific dietary allergens have been identified.<br />Objective: To compare serum immunoglobulin (Ig)E and IgG reactivity to specific food antigens in privately owned dogs with and without CAFR.<br />Animals: Eighteen adult dogs with nonseasonal pruritus recruited from a hospital population.<br />Methods and Materials: Dogs were fed an extensively hydrolysed poultry-based diet exclusively for 12 weeks. Serum was collected at the beginning of the trial. Canine atopic dermatitis extent and severity index and pruritus Visual Analog Scale scoring were performed at the beginning and end of the trial. Immunoblotting was performed to identify IgE and/or IgG binding to specific proteins in beef, egg, milk, chicken, pork, soy and wheat extracts.<br />Results: A CAFR (defined as an unequivocal relapse of pruritus after dietary challenge) was diagnosed in 10 dogs, with 60% relapsing when fed chicken-based diets. Binding of subjects' IgG to almost all proteins in all extracts was seen regardless of reported dietary history. Few proteins were exclusively or predominantly bound by IgE in CAFR dogs. Exceptions included a 42 kDa band (chicken), a 52 kDa band (beef), a 46 kDa band (beef and milk) and a poorly defined high molecular weight protein or proteins (beef and milk).<br />Conclusion: This study demonstrated three protein bands and a poorly defined band predominantly recognized by sera from dogs with CAFR relative to non-CAFR dog sera. Almost all proteins were bound by IgG in all dogs, suggesting prior exposure to unreported foods.<br /> (© 2019 ESVD and ACVD.)
- Subjects :
- Allergens blood
Animals
Dermatitis, Atopic etiology
Dermatitis, Atopic immunology
Dog Diseases etiology
Dog Diseases immunology
Dogs
Female
Food Hypersensitivity blood
Food Hypersensitivity immunology
Male
Pruritus etiology
Pruritus immunology
Severity of Illness Index
Skin immunology
Skin pathology
Allergens immunology
Animal Feed adverse effects
Dermatitis, Atopic veterinary
Food Hypersensitivity veterinary
Immunoglobulin E blood
Immunoglobulin G blood
Pruritus veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3164
- Volume :
- 31
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 31749195
- Full Text :
- https://doi.org/10.1111/vde.12810