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A redox modulated ratiometric fluorometric method based on the use of dual-color carbon dots for determination of the activity of enzymes participating in ascorbic acid-related reactions.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2019 Nov 20; Vol. 186 (12), pp. 818. Date of Electronic Publication: 2019 Nov 20. - Publication Year :
- 2019
-
Abstract
- A turn-on ratiometric fluorescent assay is described for the determination of the activity of enzymes participating in ascorbic acid-forming reactions. Blue-emitting carbon dots (bCDs; with excitation/emission wavelength at 380/450 nm) serve as fluorescent indicator. Their fluorescence is reduced by Fe <superscript>3+</superscript> ions via an inner filter effect. Yellow-emitting CDs (yCDs; with excitation/emission wavelength at 380/550 nm) serve as internal reference because their fluorescence is insensitive to Fe <superscript>3+</superscript> . Upon exposure to ascorbic acid (AA), Fe <superscript>3+</superscript> is reduced to Fe <superscript>2+</superscript> . Hence, the fluorescence of the bCDs is restored. Thus, enzymes participating in AA-related reactions such as α-glucosidase (α-Glu) and alkaline phosphatase (ALP) can be determined. α-Glu activity was quantified in the range from 0.13 to 6.70 U mL <superscript>-1</superscript> , and ALP activity was determined between 2.0 and 130 U L <superscript>-1</superscript> . Endowed with excellent sensitivity, selectivity and low background signals, the method may also be used to screen the inhibitors of α-Glu and ALP. Graphical abstractSchematic representation of a redox modulated ratiometric fluorometric method based on the use of dual-color carbon dots for determination of the activity of enzymes participating in ascorbic acid-related reactions. Blue-emitting carbon dots (bCDs) serve as fluorescent indicator while yellow-emitting CDs (yCDs) serve as internal reference.
- Subjects :
- Alkaline Phosphatase antagonists & inhibitors
Alkaline Phosphatase blood
Ascorbic Acid chemistry
Humans
Oxidation-Reduction
Particle Size
Surface Properties
alpha-Glucosidases blood
alpha-Glucosidases chemistry
Alkaline Phosphatase metabolism
Ascorbic Acid metabolism
Carbon chemistry
Color
Fluorometry
Quantum Dots chemistry
alpha-Glucosidases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 186
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 31748845
- Full Text :
- https://doi.org/10.1007/s00604-019-3820-z