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Citalopram prevents sleep-deprivation-induced reduction in CaMKII-CREB-BDNF signaling in mouse prefrontal cortex.
- Source :
-
Brain research bulletin [Brain Res Bull] 2020 Feb; Vol. 155, pp. 11-18. Date of Electronic Publication: 2019 Nov 16. - Publication Year :
- 2020
-
Abstract
- Curtailment of sleep in modern society leads to a spectrum of neuropsychiatric disorders. However, the molecular mechanisms underlying the effects of sleep deprivation (SD) remain elusive and currently there is no effective therapy to alleviate these effects. Here, we aimed to examine SD-induced cellular and molecular alterations in mouse prefrontal cortex (PFC) and whether subchronic citalopram (CTM) treatment can negate these alterations. Three-month-old C57BL/6 J mice were divided into control (Ctrl), SD, CTM alone and CTM + SD groups. CTM and CTM + SD group mice were treated with CTM for five consecutive days at a dose of 10 mg/kg per day before the experimental procedure. SD and CTM + SD group mice were sleep-deprived for 24 h using an automated treadmill method. We found that 24 h SD causes a marked reduction in the levels of phosphorylated calcium/calmodulin kinase II (pCaMKII), phosphorylated cyclic AMP-responsive element binding protein (pCREB) and brain-derived neurotrophic factor (BDNF) in mouse PFC. Patch clamp recording of pyramidal neurons from acute PFC slices revealed that SD decreases the amplitude of miniature excitatory postsynaptic currents (mEPSCs), suggesting a SD-induced postsynaptic alteration. Interestingly, subchronic CTM treatment prevents such SD-induced reductions in pCaMKII, pCREB and BDNF levels, and in mEPSC amplitude. These data suggest that CTM offers neuroprotection against SD-induced molecular and electrophysiological alterations.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Brain-Derived Neurotrophic Factor physiology
Calcium-Calmodulin-Dependent Protein Kinase Type 2 physiology
Cyclic AMP Response Element-Binding Protein physiology
Female
Male
Mice, Inbred C57BL
Miniature Postsynaptic Potentials
Pyramidal Cells drug effects
Pyramidal Cells physiology
Antidepressive Agents, Second-Generation administration & dosage
Citalopram administration & dosage
Prefrontal Cortex drug effects
Prefrontal Cortex physiopathology
Signal Transduction drug effects
Sleep Deprivation physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2747
- Volume :
- 155
- Database :
- MEDLINE
- Journal :
- Brain research bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 31743748
- Full Text :
- https://doi.org/10.1016/j.brainresbull.2019.11.007