Hepatocellular HO-1 mediated iNOS-induced hepatoprotection against liver ischemia reperfusion injury.

Authors :: Qiao Y
Xiao F
Li W
Yu M
Du P
Fang Z
Sun J
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Jan 22; Vol. 521 (4), pp. 1095-1100. Date of Electronic Publication: 2019 Nov 14.
Publication Year :: 2020
Abstract: Hepatocyte-derived inducible nitric oxide synthase (iNOS) was proved to impart protection against liver ischemia reperfusion (I/R) injury in our prior analysis. However, the mechanism for this hepatoprotection remains incompletely understood. Bone marrow chimeric mice were generated using expression of iNOS in a hepatocyte-selective manner against an iNOS-knockout background. The function of heme oxygenase 1 (HO-1) in iNOS-stimulated hepatoprotection and the molecular mechanisms were explored in vitro and in vivo, respectively. Hepatocyte-derived iNOS conferred protection from I/R injury and anoxia/reoxygenation stimulation. Mechanistically, iNOS activated nuclear factor erythroid 2-related factor 2 (Nrf-2) and subsequently, stimulated the transcription of HO-1. Results from our study led to the conclusion that HO-1 is another potent mediator of iNOS-mediated protection after liver I/R.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)

Subjects :: Animals
Cell Nucleus metabolism
Cells, Cultured
Mice, Inbred C57BL
Mice, Knockout
NF-E2-Related Factor 2 metabolism
Protein Transport
Up-Regulation genetics
Heme Oxygenase-1 metabolism
Hepatocytes enzymology
Liver pathology
Nitric Oxide Synthase Type II metabolism
Reperfusion Injury enzymology
Reperfusion Injury pathology

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