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Efficient synthesis of 5-hydroxymethyl-2-furancarboxylic acid by Escherichia coli overexpressing aldehyde dehydrogenases.
- Source :
-
Journal of biotechnology [J Biotechnol] 2020 Jan 10; Vol. 307, pp. 125-130. Date of Electronic Publication: 2019 Nov 11. - Publication Year :
- 2020
-
Abstract
- Catalytic transformation of biomass-derived furans into value-added chemicals and biofuels has received considerable interest recently. In this work, aldehyde dehydrogenases (ALDHs) were identified from Comamonas testosteroni SC1588 for the oxidation of bio-based furans into furan carboxylic acids. Of the whole-cell biocatalysts constructed, Escherichia coli expressing a vanillin dehydrogenase (E. coli&#95;CtVDH1) proved to be the best for the oxidation of 5-hydroxymethylfurfural (HMF). 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA) was obtained in the yield of approximately 92 % within 12 h using this recombinant strain when the HMF concentration was up to 200 mM. In a fed-batch process, 292 mM of HMFCA was produced within 20.5 h, thereby providing a productivity as high as 2.0 g/L h. Other furan carboxylic acids were synthesized in the yields of 83-95%. Besides, the partially purified HMF was smoothly converted into HMFCA by this recombinant strain, with a 90% yield.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Aldehyde Dehydrogenase genetics
Aldehyde Oxidoreductases genetics
Biocatalysis
Escherichia coli genetics
Escherichia coli Proteins genetics
Escherichia coli Proteins metabolism
Furaldehyde chemistry
Furaldehyde metabolism
Furans chemistry
Oxidation-Reduction
Recombinant Proteins
Aldehyde Dehydrogenase metabolism
Aldehyde Oxidoreductases metabolism
Escherichia coli enzymology
Furaldehyde analogs & derivatives
Furans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 307
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 31726082
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2019.11.007