Back to Search
Start Over
Aggregation-Related Nonphotochemical Quenching in the Photosynthetic Membrane.
- Source :
-
The journal of physical chemistry letters [J Phys Chem Lett] 2019 Dec 05; Vol. 10 (23), pp. 7340-7346. Date of Electronic Publication: 2019 Nov 14. - Publication Year :
- 2019
-
Abstract
- The photosynthetic apparatus of plants is a robust self-adjustable molecular system, able to function efficiently under varying environmental conditions. Under strong sunlight, it switches into photoprotective mode to avoid overexcitation by safely dissipating the excess absorbed light energy via nonphotochemical quenching (NPQ). Unfortunately, heterogeneous organization and simultaneous occurrence of multiple processes within the thylakoid membrane impede the study of natural NPQ under in vivo conditions; thus, usually artificially prepared antennae have been studied instead. However, it has never been shown directly that the origin of fluorescence quenching observed in these artificial systems underlies natural NPQ. Here we report the time-resolved fluorescence measurements of the dark-adapted and preilluminated-to induce NPQ-intact chloroplasts, performed over a broad temperature range. We show that their spectral response matches that observed in the LHCII aggregates, thus demonstrating explicitly for the first time that the latter in vitro system preserves essential properties of natural photoprotection.
Details
- Language :
- English
- ISSN :
- 1948-7185
- Volume :
- 10
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- The journal of physical chemistry letters
- Publication Type :
- Academic Journal
- Accession number :
- 31710503
- Full Text :
- https://doi.org/10.1021/acs.jpclett.9b03100