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Solid Phase 11C-Methylation, Purification and Formulation for the Production of PET Tracers.
- Source :
-
Journal of visualized experiments : JoVE [J Vis Exp] 2019 Oct 24 (152). Date of Electronic Publication: 2019 Oct 24. - Publication Year :
- 2019
-
Abstract
- Routine production of radiotracers used in positron emission tomography (PET) mostly relies on wet chemistry where the radioactive synthon reacts with a non-radioactive precursor in solution. This approach necessitates purification of the tracer by high performance liquid chromatography (HPLC) followed by reformulation in a biocompatible solvent for human administration. We recently developed a novel <superscript>11</superscript> C-methylation approach for the highly efficient synthesis of carbon-11 labeled PET radiopharmaceuticals, taking advantage of solid phase cartridges as disposable "3-in-1" units for the synthesis, purification and reformulation of the tracers. This approach obviates the use of preparative HPLC and reduces the losses of the tracer in transfer lines and due to radioactive decay. Furthermore, the cartridge-based technique improves synthesis reliability, simplifies the automation process and facilitates compliance with the Good Manufacturing Practice (GMP) requirements. Here, we demonstrate this technique on the example of production of a PET tracer Pittsburgh compound B ([ <superscript>11</superscript> C]PiB), a gold standard in vivo imaging agent for amyloid plaques in the human brains.
Details
- Language :
- English
- ISSN :
- 1940-087X
- Issue :
- 152
- Database :
- MEDLINE
- Journal :
- Journal of visualized experiments : JoVE
- Publication Type :
- Academic Journal
- Accession number :
- 31710043
- Full Text :
- https://doi.org/10.3791/60237