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Immunophenotypic Analysis of CAR-T Cells.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2086, pp. 195-201. - Publication Year :
- 2020
-
Abstract
- CAR-T cell immunotherapy is a promising therapeutic modality for cancer patients. The success of CAR-T cell therapy has been associated with the phenotype, activation and functional profiling of infused CAR-T cells. Therefore, immunophenotypic characterization of CAR-T cells during bioprocess is crucial for cell quality control and ultimately for improved antitumor efficacy. In this chapter, we propose a flow cytometry panel to characterize the immunophenotype of the CAR-T subsets.
- Subjects :
- Animals
Biomarkers
Cytotoxicity, Immunologic
Flow Cytometry
Humans
Immunologic Memory
Immunotherapy, Adoptive methods
Immunotherapy, Adoptive standards
Lymphocyte Activation
Mice
Receptors, Antigen, T-Cell genetics
Receptors, Chimeric Antigen genetics
T-Lymphocyte Subsets immunology
T-Lymphocyte Subsets metabolism
T-Lymphocytes immunology
Transduction, Genetic
Immunophenotyping methods
Receptors, Antigen, T-Cell metabolism
Receptors, Chimeric Antigen metabolism
T-Lymphocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2086
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 31707677
- Full Text :
- https://doi.org/10.1007/978-1-0716-0146-4_14