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Identification of a Novel Nitroreductase LNR and Its Role in Pendimethalin Catabolism in Bacillus subtilis Y3.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2019 Nov 20; Vol. 67 (46), pp. 12816-12823. Date of Electronic Publication: 2019 Nov 12. - Publication Year :
- 2019
-
Abstract
- Microbial degradation plays a major role in the dissipation of pendimethalin, and nitroreduction is an initial and detoxicating step. Previously, a pendimethalin nitroreductase, PNR, was identified in Bacillus subtilis Y3. Here, another pendimethalin nitroreductase from strain Y3, LNR, was identified. LNR shares only 40% identity with PNR and reduces the aromatic ring C-6 nitro group of pendimethalin and both nitro groups of trifluralin, butralin, and oryzalin. The catalytic activities against the four dinitroanilines were much higher for LNR than for PNR. lnr deletion significantly reduced the pendimethalin-reduction activity (60% activity loss), while pnr deletion led to only 30% activity loss, indicating that both LNR and PNR were involved in pendimethalin nitroreduction in strain Y3; however, LNR played the major role. This study facilitates the elucidation of pendimethalin catabolism and provides degrading enzyme resources for the removal of dinitroaniline herbicide residues in environment and agricultural products.
- Subjects :
- Amino Acid Sequence
Aniline Compounds chemistry
Bacillus subtilis classification
Bacillus subtilis genetics
Bacillus subtilis metabolism
Bacterial Proteins chemistry
Bacterial Proteins genetics
Biodegradation, Environmental
Herbicides chemistry
Nitroreductases chemistry
Nitroreductases genetics
Phylogeny
Sequence Alignment
Aniline Compounds metabolism
Bacillus subtilis enzymology
Bacterial Proteins metabolism
Herbicides metabolism
Nitroreductases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 67
- Issue :
- 46
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31675231
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b04354