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Antiproliferative Pterocarpans and Coumestans from Lespedeza bicolor .
- Source :
-
Journal of natural products [J Nat Prod] 2019 Nov 22; Vol. 82 (11), pp. 3025-3032. Date of Electronic Publication: 2019 Nov 01. - Publication Year :
- 2019
-
Abstract
- Chromatographic purification of a methanol extract of the roots of Lespedeza bicolor led to the isolation of four new pterocarpans ( 1 - 4 ), two new coumestans ( 6 and 7 ), two new arylbenzofurans ( 8 and 9 ), and the known pterocarpan 1-methoxyerythrabyssin II ( 5 ). Their structures were identified using NMR spectroscopy, UV spectroscopy, and mass spectrometry. Cytotoxicity assays showed that compounds 1 - 9 exerted antiproliferative effects on blood cancer cells. Of these compounds, 1 and 6 induced mitochondrial depolarization and induced apoptosis in Jurkat cells. These compounds promoted cell death by inducing cell-cycle arrest at the G1 stage, reducing levels of BCL2, and increasing cleavage of PARP-1. These findings indicate that 1 and 6 are possible lead compounds for the treatment of human leukemia cells via intracellular signaling.
- Subjects :
- Antineoplastic Agents, Phytogenic isolation & purification
Apoptosis drug effects
Cell Cycle Checkpoints drug effects
Cell Line, Tumor
Cell Survival drug effects
Coumarins isolation & purification
Hematologic Neoplasms blood
Hematologic Neoplasms drug therapy
Humans
Jurkat Cells
Magnetic Resonance Spectroscopy
Mitochondria drug effects
Molecular Structure
Pterocarpans isolation & purification
Spectrophotometry, Ultraviolet
Antineoplastic Agents, Phytogenic pharmacology
Coumarins pharmacology
Lespedeza chemistry
Pterocarpans pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6025
- Volume :
- 82
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of natural products
- Publication Type :
- Academic Journal
- Accession number :
- 31675225
- Full Text :
- https://doi.org/10.1021/acs.jnatprod.9b00567